Review



pet28b e1  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc pet28b e1
    Pet28b E1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/10__7554_slash_elife__76100-347-20-39
    Average 90 stars, based on 4 article reviews
    pet28b e1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9
    Article Snippet: To pSUMO1 (gift from Primo Schaer, Addgene #52258),53 genes of codon-optimized Ub with an N-terminal TEV-cleavable His6 tag, codon-optimized Ubc9 K14R and ChE1 v4.5 were cloned after each of the three operons.

    Clone Assay:

    Article Title: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9
    Article Snippet: To pSUMO1 (gift from Primo Schaer, Addgene #52258),53 genes of codon-optimized Ub with an N-terminal TEV-cleavable His6 tag, codon-optimized Ubc9 K14R and ChE1 v4.5 were cloned after each of the three operons.

    Plasmid Preparation:

    Article Title: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9
    Article Snippet: To pSUMO1 (gift from Primo Schaer, Addgene #52258),53 genes of codon-optimized Ub with an N-terminal TEV-cleavable His6 tag, codon-optimized Ubc9 K14R and ChE1 v4.5 were cloned after each of the three operons.



    Similar Products

    pet28b e1  (Addgene inc)
    90
    Addgene inc pet28b e1
    Pet28b E1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/10__7554_slash_elife__76100-347-20-39
    Average 90 stars, based on 1 article reviews
    pet28b e1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    pgex6p1 ube2s ubd  (Addgene inc)
    90
    Addgene inc pgex6p1 ube2s ubd
    Pgex6p1 Ube2s Ubd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/pmc08883482__oc1c01490_si_001-100-10-15
    Average 90 stars, based on 1 article reviews
    pgex6p1 ube2s ubd - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ubiquitin conjugating e2 enzyme ube2s  (Addgene inc)
    90
    Addgene inc ubiquitin conjugating e2 enzyme ube2s
    Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and <t>UBE2S</t> (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2
    Ubiquitin Conjugating E2 Enzyme Ube2s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/pmc07236025-102-8-13
    Average 90 stars, based on 1 article reviews
    ubiquitin conjugating e2 enzyme ube2s - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    pgex-6p  (Addgene inc)
    90
    Addgene inc pgex-6p
    Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and <t>UBE2S</t> (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2
    Pgex 6p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/bio_rxiv__2020__02__21__960088-216-27-51
    Average 90 stars, based on 1 article reviews
    pgex-6p - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and UBE2S (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2

    Journal: Journal of mass spectrometry : JMS

    Article Title: Top-down analysis of novel synthetic branched proteins

    doi: 10.1002/jms.4303

    Figure Lengend Snippet: Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and UBE2S (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2

    Article Snippet: Rub1 T72R (10 mg) was incubated with 20-μM ubiquitin-conjugating E2 enzyme UBE2S (UBE2S-UBD, Addgene #66713), 500-nM UBE1, 10 mM creatine phosphate, 5 mM MgCl 2 , 5 mM ATP, and creatine phosphokinase in 50-mM Tris-HCl buffer (pH 8) for approximately 16 hours.

    Techniques: Staining, SDS Page, Ubiquitin Proteomics, Centrifugation